Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features.

In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α).

Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes;

and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo.

—moraldo turkey

In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactivecompounds potentially able to protect vascular system against various stressors.

HemoVoid Buffer Kit - 500

HVBK-500 500 mL
EUR 615
Description: HemoVoid™ Buffer

AlbuVoid Buffer Kit - 500

AVBK-500 500 mL
EUR 615
Description: Albuvoid™ Buffer

ERLENMEYER FLASK, 500 ML, PP

4985P-500 6/pk
EUR 64
Description: Reusable Plastics; Reusable Erl Flasks

ERLENMEYER FLASK, 500 ML, PMP

4990P-500 6/pk
EUR 92
Description: Reusable Plastics; Reusable Erl Flasks

WASH BOTTLE 500 ML, LDPE

1300P-500 12/pk
EUR 81
Description: Reusable Plastics; Resusable Bottles

REAGENT BOTTLE,500 ML, PP

1500P-500 10/pk
EUR 62
Description: Reusable Plastics; Resusable Bottles

DBCO-PEG-Biotin,500

HE048041-500-100mg 100mg
EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

PLGA(50:50)?8K?-PEG-Mal,500

HE086022-500-500mg 500mg
EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Alkyne CPG modifier 500, 500 mg

32860 500 mg
EUR 193

miRZip-500 anti-miR-500 microRNA construct

MZIP500-PA-1 Bacterial Streak
EUR 684

FAM CPG 500, 6-isomer, 500 mg

35760 500 mg
EUR 249

TFA-Amino modifier CPG 500, 500 mg

31860 500 mg
EUR 193

Measuring Spoon 500

MSP05 25pack
EUR 123
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes.

ZeptoGel (500 mL)

0801195 500 mL
EUR 327.68
Description: Please contact Gentaur in order to receive the datasheet of the product.

Cellufine A-500

19805 500 ml
EUR 526

Cellufine A-500

19806 5 lt Ask for price

Cellufine C-500

19865 500 ml
EUR 526

Cellufine C-500

19866 5 lt Ask for price

Cellufine Q-500

19907 500 ml
EUR 718

Cellufine Q-500

19908 5 lt Ask for price

Cellufine S-500

21-200 100 ml
EUR 388

Cellufine S-500

21-201 500 ml
EUR 697

Cellufine S-500

21-202 5 lt Ask for price

Cellufine S-500

21-203 10 lt Ask for price

Cellufine A-500

675980327 100 ml
EUR 324

Cellufine A-500

675980335 10 lt Ask for price

Cellufine Q-500

675982327 100 ml
EUR 366

Cellufine Q-500

675982335 10 lt Ask for price

Cellufine C-500

675983327 100 ml
EUR 324

Cellufine C-500

675983335 10 lt Ask for price

mPEG-SH,500

33-MF001003-500
  • EUR 259.00
  • EUR 677.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-COOH?500

33-MF001017-500
  • EUR 198.00
  • EUR 494.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-MAL,500

33-MF001022-500
  • EUR 235.00
  • EUR 605.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

IMDM, 500 ml

411 500 ml
EUR 89

500 ML SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS

4500-500 1/pk
EUR 529
Description: Bioprocess Vessels; Spinner Flask 70

TUBE,MICENT,1.7ML,NAT,SC,NS,BK,500/500

3620 500/pk
EUR 56
Description: Microcentrifuge Tubes; Snap Cap Tubes

cleaning solution 500 ml

B530 ea
EUR 30

500 G HEPES, POWDER

61-034-RO 500 g/pk
EUR 146
Description: Media Catalog; Cell Culture Reagents

PLASTIC CLAMP, 500 ML

480151 1/pk
EUR 70
Description: Lab Equipment; Constant Temperature Equipment

Silicone oil, 500 cSt

GK1916-1L 1 l
EUR 150

Silicone oil, 500 cSt

GK1916-250ML 250 ml
EUR 78

MC Cellufine C-500

19-800-51 5 x 1 ml
EUR 217

MC Cellufine C-500

19-800-55 5 x 5 ml
EUR 345

MC Cellufine A-500

19-805-51 5 x 1 ml
EUR 217

MC Cellufine A-500

19-805-55 5 x 5 ml
EUR 345

MC Cellufine Q-500

19-907-51 5 x 1 ml
EUR 217

MC Cellufine Q-500

19-907-55 5 x 5 ml
EUR 345

MC Cellufine S-500

21-200-51 5 x 1 ml
EUR 239

MC Cellufine S-500

21-200-55 5 x 5 ml
EUR 420

Hinc II unit: 500

YRHINC2 1 vial Ask for price

Nru I unit: 500

YRNRU1 1 vial Ask for price

HO-PEG-COOH,500

33-HE002017-500
  • EUR 198.00
  • EUR 494.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-COOH,500

33-HE003017-500
  • EUR 519.00
  • EUR 1456.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

HS-PEG-NHS,500

33-HE003023-500
  • EUR 445.00
  • EUR 1234.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-COOH,500

33-HE005017-500
  • EUR 359.00
  • EUR 975.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-OH,500

33-HE006002-500
  • EUR 568.00
  • EUR 1603.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-BIOTIN,500

33-HE006041-500
  • EUR 827.00
  • EUR 2380.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Biotin-PEG-COOH,500

33-HE041017-500
  • EUR 605.00
  • EUR 1714.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-SH,500

33-HO003003-500
  • EUR 259.00
  • EUR 677.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

AlKyne-PEG-AlKyne,500

33-HO007007-500
  • EUR 283.00
  • EUR 747.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

COOH-PEG-COOH,500

33-HO017017-500
  • EUR 167.00
  • EUR 399.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SC-PEG-SC,500

33-HO023023-500
  • EUR 167.00
  • EUR 399.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cal-500™ AM

20412 10x50 ug
EUR 219

Protonex™ Green 500

21215 1 mg
EUR 219

BMZERO™, 500 ml

401 500 ml
EUR 105

EPM2™, 500 ml

402 500 ml
EUR 107

HPC1™, 500 ml

403 500 ml
EUR 110

Refolding Buffers - 500 ml

612 500 ml
EUR 247

Augmedium™, Powder 500

124 500 ml
EUR 566

mmu-miR-500 Primers

MPM00559 150 ul / 10 uM
EUR 121

rno-miR-500 Primers

MP-r00247 150 ul / 10 uM
EUR 176

THPTA ligand, 500 mg

H4050 500 mg
EUR 216

electrolyte 500 ml 4m licl

B525 ea
EUR 121

Compact Balance, 500 grams, 115V

BCM1302 1 pcs, 1 UNIT
EUR 456.19

Precision Balance, 500 grams, 115V

BCM1308 1 pcs, 1 UNIT
EUR 1479.17

Measuring Spoon 500 RNase Free

MSP05-RNA 10pack
EUR 136
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes. RNase free.

DiagNano Silica Particles, 500 nm

DNG-B009 10 mL
EUR 559

T4 DNA Polymerase - 500 units

3222 1/EA
EUR 206

500 ML HYBRIGRO SF™

40-215-CVR 500 mL/pk
EUR 200
Description: Specialty Media; Serum Free and Specialty Media

500 G L-GLUTAMINE, POWDER

61-030-RO 500 g/pk
EUR 199
Description: Media Catalog; Cell Culture Reagents

500 G POLYSUCROSE 400, POWDER

61-196-RO 500 g/pk
EUR 507
Description: Specialty Media; Insect Media Products

500 G YEAST EXTRACT, POWDER

61-231-RO 500 g/pk
EUR 178
Description: Media Catalog; Cell Culture Reagents

BioRad CFX96, Megabase 500/1000

A28241L 5x25 pcs
EUR 303

500 ML SODIUM CHLORIDE, 5M

46-032-CV 500 mL/pk
EUR 90
Description: Media Catalog; Classical Media

500 ML MCDB 131, 1X

15-100-CV 500 mL/pk
EUR 132
Description: Media Catalog; Classical Media

SharpProtein MarkerII unit: 500 µl

FYP005-500UL 1 vial Ask for price

Polyclonal CDC6 Antibody (aa400-500)

APR02453G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CDC6 (aa400-500). This antibody is tested and proven to work in the following applications:

Polyclonal ZBTB2 Antibody (aa450-500)

APR02982G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ZBTB2 (aa450-500). This antibody is tested and proven to work in the following applications:

mFluor™ Violet 500 SE

1149 1 mg
EUR 219

Cal-500™, potassium salt

20410 10x50 ug
EUR 219

Protonex™ Green 500, SE

21216 1 mg
EUR 219

Protonex™ Green 500 Dextran

21217 1 mg
EUR 219

P4-8F™, 500 ml

404 500 ml
EUR 107

LB*Booster™, Powder 500

126 500 ml
EUR 190

APF LB Lennox, 500 g

132 500 g
EUR 107

mmu-miR-500-3p Primers

MPM01366 150 ul / 10 uM
EUR 121