Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features.

In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α).

Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes;

and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo.

—moraldo turkey

In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactivecompounds potentially able to protect vascular system against various stressors.

HemoVoid Buffer Kit - 500
HVBK-500	Biotech Support Group	500 mL	EUR 615
Description: HemoVoid™ Buffer

AlbuVoid Buffer Kit - 500
AVBK-500	Biotech Support Group	500 mL	EUR 615
Description: Albuvoid™ Buffer

ERLENMEYER FLASK, 500 ML, PP
4985P-500	CORNING	6/pk	EUR 64
Description: Reusable Plastics; Reusable Erl Flasks

ERLENMEYER FLASK, 500 ML, PMP
4990P-500	CORNING	6/pk	EUR 92
Description: Reusable Plastics; Reusable Erl Flasks

WASH BOTTLE 500 ML, LDPE
1300P-500	CORNING	12/pk	EUR 81
Description: Reusable Plastics; Resusable Bottles

REAGENT BOTTLE,500 ML, PP
1500P-500	CORNING	10/pk	EUR 62
Description: Reusable Plastics; Resusable Bottles

DBCO-PEG-Biotin,500
HE048041-500-100mg	Biochempeg	100mg	EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

PLGA(50:50)?8K?-PEG-Mal,500
HE086022-500-500mg	Biochempeg	500mg	EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Alkyne CPG modifier 500, 500 mg
32860	Lumiprobe	500 mg	EUR 193

miRZip-500 anti-miR-500 microRNA construct
MZIP500-PA-1	SBI	Bacterial Streak	EUR 684

FAM CPG 500, 6-isomer, 500 mg
35760	Lumiprobe	500 mg	EUR 249

TFA-Amino modifier CPG 500, 500 mg
31860	Lumiprobe	500 mg	EUR 193

Measuring Spoon 500
MSP05	Next Advance	25pack	EUR 123
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes.

ZeptoGel (500 mL)
0801195	Zeptometrix	500 mL	EUR 327.68
Description: Please contact Gentaur in order to receive the datasheet of the product.

Cellufine A-500
19805	JNC America	500 ml	EUR 526

Cellufine A-500
19806	JNC America	5 lt	Ask for price

Cellufine C-500
19865	JNC America	500 ml	EUR 526

Cellufine C-500
19866	JNC America	5 lt	Ask for price

Cellufine Q-500
19907	JNC America	500 ml	EUR 718

Cellufine Q-500
19908	JNC America	5 lt	Ask for price

Cellufine S-500
21-200	JNC America	100 ml	EUR 388

Cellufine S-500
21-201	JNC America	500 ml	EUR 697

Cellufine S-500
21-202	JNC America	5 lt	Ask for price

Cellufine S-500
21-203	JNC America	10 lt	Ask for price

Cellufine A-500
675980327	JNC America	100 ml	EUR 324

Cellufine A-500
675980335	JNC America	10 lt	Ask for price

Cellufine Q-500
675982327	JNC America	100 ml	EUR 366

Cellufine Q-500
675982335	JNC America	10 lt	Ask for price

Cellufine C-500
675983327	JNC America	100 ml	EUR 324

Cellufine C-500
675983335	JNC America	10 lt	Ask for price

mPEG-SH,500
33-MF001003-500	Biochempeg	EUR 259.00 EUR 677.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-COOH?500
33-MF001017-500	Biochempeg	EUR 198.00 EUR 494.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-MAL,500
33-MF001022-500	Biochempeg	EUR 235.00 EUR 605.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

IMDM, 500 ml
411	AthenaES	500 ml	EUR 89

500 ML SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS
4500-500	CORNING	1/pk	EUR 529
Description: Bioprocess Vessels; Spinner Flask 70

TUBE,MICENT,1.7ML,NAT,SC,NS,BK,500/500
3620	CORNING	500/pk	EUR 56
Description: Microcentrifuge Tubes; Snap Cap Tubes

cleaning solution 500 ml
B530	Consort	ea	EUR 30

500 G HEPES, POWDER
61-034-RO	CORNING	500 g/pk	EUR 146
Description: Media Catalog; Cell Culture Reagents

PLASTIC CLAMP, 500 ML
480151	CORNING	1/pk	EUR 70
Description: Lab Equipment; Constant Temperature Equipment

Silicone oil, 500 cSt
GK1916-1L	Glentham Life Sciences	1 l	EUR 150

Silicone oil, 500 cSt
GK1916-250ML	Glentham Life Sciences	250 ml	EUR 78

MC Cellufine C-500
19-800-51	JNC America	5 x 1 ml	EUR 217

MC Cellufine C-500
19-800-55	JNC America	5 x 5 ml	EUR 345

MC Cellufine A-500
19-805-51	JNC America	5 x 1 ml	EUR 217

MC Cellufine A-500
19-805-55	JNC America	5 x 5 ml	EUR 345

MC Cellufine Q-500
19-907-51	JNC America	5 x 1 ml	EUR 217

MC Cellufine Q-500
19-907-55	JNC America	5 x 5 ml	EUR 345

MC Cellufine S-500
21-200-51	JNC America	5 x 1 ml	EUR 239

MC Cellufine S-500
21-200-55	JNC America	5 x 5 ml	EUR 420

Hinc II unit: 500
YRHINC2	Yeastern Biotech	1 vial	Ask for price

Nru I unit: 500
YRNRU1	Yeastern Biotech	1 vial	Ask for price

HO-PEG-COOH,500
33-HE002017-500	Biochempeg	EUR 198.00 EUR 494.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-COOH,500
33-HE003017-500	Biochempeg	EUR 519.00 EUR 1456.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

HS-PEG-NHS,500
33-HE003023-500	Biochempeg	EUR 445.00 EUR 1234.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-COOH,500
33-HE005017-500	Biochempeg	EUR 359.00 EUR 975.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-OH,500
33-HE006002-500	Biochempeg	EUR 568.00 EUR 1603.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-BIOTIN,500
33-HE006041-500	Biochempeg	EUR 827.00 EUR 2380.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Biotin-PEG-COOH,500
33-HE041017-500	Biochempeg	EUR 605.00 EUR 1714.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-SH,500
33-HO003003-500	Biochempeg	EUR 259.00 EUR 677.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

AlKyne-PEG-AlKyne,500
33-HO007007-500	Biochempeg	EUR 283.00 EUR 747.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

COOH-PEG-COOH,500
33-HO017017-500	Biochempeg	EUR 167.00 EUR 399.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SC-PEG-SC,500
33-HO023023-500	Biochempeg	EUR 167.00 EUR 399.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cal-500™ AM
20412	AAT Bioquest	10x50 ug	EUR 219

Protonex™ Green 500
21215	AAT Bioquest	1 mg	EUR 219

BMZERO™, 500 ml
401	AthenaES	500 ml	EUR 105

EPM2™, 500 ml
402	AthenaES	500 ml	EUR 107

HPC1™, 500 ml
403	AthenaES	500 ml	EUR 110

Refolding Buffers - 500 ml
612	AthenaES	500 ml	EUR 247

Augmedium™, Powder 500
124	AthenaES	500 ml	EUR 566

mmu-miR-500 Primers
MPM00559	ABM	150 ul / 10 uM	EUR 121

rno-miR-500 Primers
MP-r00247	ABM	150 ul / 10 uM	EUR 176

THPTA ligand, 500 mg
H4050	Lumiprobe	500 mg	EUR 216

electrolyte 500 ml 4m licl
B525	Consort	ea	EUR 121

Compact Balance, 500 grams, 115V
BCM1302	Bio Basic	1 pcs, 1 UNIT	EUR 456.19

Precision Balance, 500 grams, 115V
BCM1308	Bio Basic	1 pcs, 1 UNIT	EUR 1479.17

Measuring Spoon 500 RNase Free
MSP05-RNA	Next Advance	10pack	EUR 136
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes. RNase free.

DiagNano Silica Particles, 500 nm
DNG-B009	Creative Diagnostics	10 mL	EUR 559

T4 DNA Polymerase - 500 units
3222	Intact Genomics	1/EA	EUR 206

500 ML HYBRIGRO SF™
40-215-CVR	CORNING	500 mL/pk	EUR 200
Description: Specialty Media; Serum Free and Specialty Media

500 G L-GLUTAMINE, POWDER
61-030-RO	CORNING	500 g/pk	EUR 199
Description: Media Catalog; Cell Culture Reagents

500 G POLYSUCROSE 400, POWDER
61-196-RO	CORNING	500 g/pk	EUR 507
Description: Specialty Media; Insect Media Products

500 G YEAST EXTRACT, POWDER
61-231-RO	CORNING	500 g/pk	EUR 178
Description: Media Catalog; Cell Culture Reagents

BioRad CFX96, Megabase 500/1000
A28241L	GeneOn	5x25 pcs	EUR 303

500 ML SODIUM CHLORIDE, 5M
46-032-CV	CORNING	500 mL/pk	EUR 90
Description: Media Catalog; Classical Media

500 ML MCDB 131, 1X
15-100-CV	CORNING	500 mL/pk	EUR 132
Description: Media Catalog; Classical Media

SharpProtein MarkerII unit: 500 µl
FYP005-500UL	Yeastern Biotech	1 vial	Ask for price

Polyclonal CDC6 Antibody (aa400-500)
APR02453G	Leading Biology	0.05ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CDC6 (aa400-500). This antibody is tested and proven to work in the following applications:

Polyclonal ZBTB2 Antibody (aa450-500)
APR02982G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ZBTB2 (aa450-500). This antibody is tested and proven to work in the following applications:

mFluor™ Violet 500 SE
1149	AAT Bioquest	1 mg	EUR 219

Cal-500™, potassium salt
20410	AAT Bioquest	10x50 ug	EUR 219

Protonex™ Green 500, SE
21216	AAT Bioquest	1 mg	EUR 219

Protonex™ Green 500 Dextran
21217	AAT Bioquest	1 mg	EUR 219

P4-8F™, 500 ml
404	AthenaES	500 ml	EUR 107

LB*Booster™, Powder 500
126	AthenaES	500 ml	EUR 190

APF LB Lennox, 500 g
132	AthenaES	500 g	EUR 107

mmu-miR-500-3p Primers
MPM01366	ABM	150 ul / 10 uM	EUR 121